Monash University TARDIS DatasetPackager v1.0 Crystal structure of wildtype MENT in the native conformation 2H4R experiment Primary Citation http://www.rcsb.org/pdb/explore.do?structureId=2H4R Most serpins are associated with protease inhibition, and their ability to form loop-sheet polymers is linked to conformational disease and the human serpinopathies. Here we describe the structural and functional dissection of how a unique serpin, the non-histone architectural protein, MENT (Myeloid and Erythroid Nuclear Termination stage-specific protein), participates in DNA and chromatin condensation. Our data suggest that MENT contains at least two distinct DNA-binding sites, consistent with its simultaneous binding to the two closely juxtaposed linker DNA segments on a nucleosome. Remarkably, our studies suggest that the reactive centre loop, a region of the MENT molecule essential for chromatin bridging in vivo and in vitro, is able to mediate formation of a loop-sheet oligomer. These data provide mechanistic insight into chromatin compaction by a non-histone architectural protein and suggest how the structural plasticity of serpins has adapted to mediate physiological, rather than pathogenic, loop-sheet linkages. Buckle A.M. author Irving J.A. author Whisstock J.C. author Native Diffraction Intensities internal J-1 level 1 Representation 2.7 Rotating Anode RIGAKU RAXIS IV MENT WT R-AXIS 35.0 35.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 35.5 36.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 36.0 36.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 36.5 37.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 37.0 37.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 37.5 38.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 38.0 38.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 38.5 39.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 39.0 39.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 39.5 40.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 40.0 40.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 40.5 41.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 41.0 41.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 41.5 42.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 42.0 42.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 42.5 43.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 43.0 43.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 43.5 44.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 44.0 44.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 44.5 45.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 45.0 45.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 45.5 46.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 46.0 46.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 46.5 47.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 47.0 47.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 47.5 48.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 48.0 48.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 48.5 49.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 49.0 49.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 49.5 50.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 50.0 50.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 50.5 51.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 51.0 51.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 51.5 52.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 52.0 52.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 52.5 53.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 53.0 53.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 53.5 54.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 54.0 54.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 54.5 55.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 55.0 55.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 55.5 56.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 56.0 56.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 56.5 57.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 57.0 57.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 57.5 58.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 58.0 58.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 58.5 59.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 59.0 59.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 59.5 60.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 60.0 60.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 60.5 61.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 61.0 61.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 61.5 62.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 62.0 62.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 62.5 63.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 63.0 63.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 63.5 64.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 64.0 64.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 64.5 65.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 65.0 65.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 65.5 66.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 66.0 66.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 66.5 67.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 67.0 67.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 67.5 68.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 68.0 68.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 68.5 69.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 69.0 69.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 69.5 70.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 70.0 70.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 70.5 71.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 71.0 71.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 71.5 72.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 72.0 72.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 72.5 73.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 73.0 73.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 73.5 74.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 74.0 74.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 74.5 75.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 75.0 75.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 75.5 76.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 76.0 76.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 76.5 77.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 77.0 77.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 77.5 78.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 78.0 78.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 78.5 79.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 79.0 79.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 79.5 80.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 80.0 80.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 80.5 81.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 81.0 81.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 81.5 82.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 82.0 82.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 82.5 83.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 83.0 83.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 83.5 84.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 84.0 84.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 84.5 85.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 85.0 85.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 85.5 86.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 86.0 86.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 86.5 87.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 87.0 87.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 87.5 88.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 88.0 88.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 88.5 89.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 89.0 89.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 89.5 90.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 90.0 90.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 90.5 91.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 91.0 91.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 91.5 92.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 92.0 92.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 92.5 93.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 93.0 93.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 93.5 94.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 94.0 94.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 94.5 95.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 95.0 95.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 95.5 96.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 96.0 96.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 96.5 97.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 97.0 97.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 97.5 98.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 98.0 98.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 98.5 99.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 99.0 99.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 99.5 100.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 100.0 100.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 100.5 101.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 101.0 101.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 101.5 102.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 102.0 102.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 102.5 103.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 103.0 103.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 103.5 104.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 104.0 104.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 104.5 105.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 105.0 105.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 105.5 106.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 106.0 106.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 106.5 107.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 107.0 107.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 107.5 108.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 108.0 108.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 108.5 109.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 109.0 109.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 109.5 110.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 110.0 110.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 110.5 111.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 111.0 111.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 111.5 112.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 112.0 112.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 112.5 113.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 113.0 113.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 113.5 114.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 114.0 114.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 114.5 115.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 115.0 115.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 115.5 116.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 116.0 116.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 116.5 117.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 117.0 117.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 117.5 118.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 118.0 118.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 118.5 119.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 119.0 119.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 119.5 120.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 120.0 120.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 120.5 121.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 121.0 121.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 121.5 122.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 122.0 122.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 122.5 123.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 123.0 123.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 123.5 124.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 124.0 124.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 124.5 125.0 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 125.0 125.5 3.5166667 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 125.5 126.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 126.0 126.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 126.5 127.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 127.0 127.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 127.5 128.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 128.0 128.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 128.5 129.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 129.0 129.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 129.5 130.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 130.0 130.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 130.5 131.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 131.0 131.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 131.5 132.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 132.0 132.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 132.5 133.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 133.0 133.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 133.5 134.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 134.0 134.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 134.5 135.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 135.0 135.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 135.5 136.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 136.0 136.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 136.5 137.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 137.0 137.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 137.5 138.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 138.0 138.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 138.5 139.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 139.0 139.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 139.5 140.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 140.0 140.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 140.5 141.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 141.0 141.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 141.5 142.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 142.0 142.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 142.5 143.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 143.0 143.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 143.5 144.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 144.0 144.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 144.5 145.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 145.0 145.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 145.5 146.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 146.0 146.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 146.5 147.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 147.0 147.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 147.5 148.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 148.0 148.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 148.5 149.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 149.0 149.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 149.5 150.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 150.0 150.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 150.5 151.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 151.0 151.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 151.5 152.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 152.0 152.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 152.5 153.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 153.0 153.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 153.5 154.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 154.0 154.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 154.5 155.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 155.0 155.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 155.5 156.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 156.0 156.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 156.5 157.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 157.0 157.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 157.5 158.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 158.0 158.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 158.5 159.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 159.0 159.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 159.5 160.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 160.0 160.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 160.5 161.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 161.0 161.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 161.5 162.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 162.0 162.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 162.5 163.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 163.0 163.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 163.5 164.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 164.0 164.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 164.5 165.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 165.0 165.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 165.5 166.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 166.0 166.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 166.5 167.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 167.0 167.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 167.5 168.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 168.0 168.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 168.5 169.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 169.0 169.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 169.5 170.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 170.0 170.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 170.5 171.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 171.0 171.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 171.5 172.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 172.0 172.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 172.5 173.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 173.0 173.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 173.5 174.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 174.0 174.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 174.5 175.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 175.0 175.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 175.5 176.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 176.0 176.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 176.5 177.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 177.0 177.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 177.5 178.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 178.0 178.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 178.5 179.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 179.0 179.5 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0 R-AXIS 179.5 180.0 4.5 N/A 1.5418 149.59 150.5 220.0 3000.0 3000.0 0.1 0.1 0.0